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KMID : 0382619890090020441
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Hanyang Journal of Medicine
1989 Volume.9 No. 2 p.441 ~ p.452
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Isolation and Nature of Proteins Specific to Epidermoid Cancer of Lung
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Abstract
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In order to investigate whether proteins specific to epidermoid cancer of the lung was present in the lung cancer tissue, proteins of the lung tissues with epidermoid cancer were isolated and fractionated by a DEAE-cellulose column chromatography, phosphocellulose column chromatography, high performance liquid chromatography (HPLC) and polyacrylamide gel electrophoresis (PAGE) and were compared with those of the control lung tissues. Also studied was nature of proteins isolated from the epidermoid cancer tissues of the lung.
1. Protein concentration of the epidermoid cancer tissue of the lung was unchanged.
2. Proteins in the epidermoid cancer tissue of the lung was isolated by a DEAE-
cellulose column chromatography into 7 peaks. One of the protein peaks (peak VIII)
present in the control lung tissue was absent in the lung cancer.
3. DEAE peak V proteins isolated from the epidermoid cancer tissue of the lung was further fractionated by a phosphocellulose column chromatography into 3 subpeaks. Two of the protein subpeaks (subpeak IV-1 and V-2) present in the DEAE peak V from the control lung tissue was suppressed in the lung cancer tissue.
4. DEAE peak I and peak IV proteins isolated from the epidermoid cancer tissue of the lung was further fractionated by a HPLC into 5 subpeaks each. One of the protein subpeaks (subpeak I-4) present in DEAE peak I from the control lung tissue disappeared in the lung cancer tissue. The ratio of RNase subpeak/non-RNase subpeak of subpeak proteins isolated from DEAE peak I and peak IV were found to be higher in the lung cancer tissue than in the control lung tissue.
5. DEAE peak I and peak IV proteins isolated from the epidermoid cancer tissue of the lung was further separated into four bands each. Protein bands 5 and 7 from DEAE peak I and bands 6 and 7 from DEAE peak IV present in the control lung tissue were not found in the epidermoid cancer tissue of the lung.
6. All of the protein peaks or subpeaks suppressed in the epidermoid cancer tissue of the lung were observed to be non-RNase proteins in nature.
The results indicated that proteins specific to the epidermoid cancer of the lung were not found in the lung cancer tissue, but a part of proteins present in the control lung tissue were suppressed in multiple in the lung cancer tissue and that all of the proteins suppressed were non-RNase in nature.
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